By Roger Narayan, Paolo Colombo, Dileep Singh, Jonathan Salem
Increase your figuring out within the most dear points of advances in bioceramics and porous ceramics. This choice of logically equipped and punctiliously chosen articles include the lawsuits of the “Porous Ceramics: Novel advancements and purposes” and “Next new release Bioceramics” symposia, which have been hung on January 27-February 1, 2008.
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Additional info for Advances in Bioceramics and Porous Ceramics II
Fluorescence was then measured with an excitation wavelength at 488 nm and an emission wavelength at 525 nm using a SpectraMax@ fluorescence microplate reader (Molecular Devices, Sunnyvale, CA). For the attachment studies, culture media was removed from each plate containing scaffolds and the control plates after 1 and 3 days of incubation as described above. The scaffolds with the stability rings were moved to empty wells, whereas the control plates were examined directly to determine the degree of non-specific binding to the plates.
The scaffolds with the stability rings were moved to empty wells, whereas the control plates were examined directly to determine the degree of non-specific binding to the plates. After adding 1 mL of a Tris buffer (10 mM Tris-CI, 1 mM EDTA) to each well, the plates were sealed and the cells were lysed through two freezeithaw cycles. Then, 100 pL of the PicoGreen@reagent was added to 100 pL of the cell lysate in a 96-well plate before measuring fluorescence in the fluorescence microplate reader [ 161.
EXPERIMENTAL PROCEDURE Pure-HA was synthesized by a precipitation method [22-241. 4H20) and di-ammonium hydrogen phosphate ((NH4)2HP04) were mixed into each other to obtain pure HA. 3 M (NH&HP04 was separately prepared in distilled water. 67 to obtain the correct stoichiometry of HA. Ammonia solution was added into both solutions to bring the pH to 11-12 level. Calcium nitrate solution was dropwisely added into continuously stirring di-ammonium hydrogen phosphate solution. During stirring, the mixture was initially heated until boiling in order to increase the reaction rate and it was left to stirring overnight at room temperature (RT).
Advances in Bioceramics and Porous Ceramics II by Roger Narayan, Paolo Colombo, Dileep Singh, Jonathan Salem